Atlas
Chemistry / Electrolytes

BicarbonateHCO₃⁻

Principal extracellular buffer; the metabolic axis of acid-base.

Sample
Serum (calculated from total CO₂ on chemistry panels)
Clinical reference range
22 to 29 mmol/L
Adult
Conv: 2229 mmol/L
Biology

What it measures

Generated by renal H⁺ secretion and consumed buffering acid loads. Directly proportional to pH on the Henderson-Hasselbalch equation.

Clinical use

Why we order it

Detect metabolic acidosis (low) or alkalosis (high); guides further ABG interpretation.

Lens · Clinical

Interpretation by lens

Clinical interpretation

Always pair with anion gap; isolated low HCO₃ with normal AG points to GI or renal HCO₃ loss.

Differential

Causes of abnormal values

Causes of HIGH
  • Vomiting
  • Diuretics
  • Hyperaldosteronism
  • Compensated respiratory acidosis
Causes of LOW
  • DKA, lactic acidosis, salicylate, methanol/ethylene glycol (high AG)
  • Diarrhea, RTA (normal AG)
  • Compensated respiratory alkalosis
Pitfalls

Pre-analytic & interpretation traps

  • !Total CO₂ on a venous chemistry panel underestimates true HCO₃ by ~2 mmol/L.
Related

Related biomarkers