Atlas
Iron studies

Ferritin

Iron-storage protein; the single best marker of body iron stores — but also an acute-phase reactant.

Sample
Serum or plasma
Fasting
Not strictly required; some functional clinicians prefer fasting morning draws for reproducibility.
Half-life
30 hours (intravascular)
Clinical reference range
30 to 400 µg/L
Adult male
Conv: 30400 µg/LSI: 13150 µg/LFunc: 50150 µg/L
Biology

What it measures

Ferritin sequesters Fe(III) in a hollow protein shell (up to 4500 Fe atoms). Serum ferritin is mostly secreted by macrophages and hepatocytes in proportion to intracellular stores — except during inflammation, when synthesis is upregulated by IL-6.

Clinical use

Why we order it

Diagnose iron deficiency (most specific marker), screen for iron overload (HFE-related hemochromatosis, transfusional siderosis), and monitor therapy.

Lens · Clinical

Interpretation by lens

Clinical interpretation

In symptomatic patients with ferritin <30 µg/L, treat empirically with iron and investigate cause concurrently. Above 1000 µg/L, work up overload, inflammation, and liver disease.

Functional interpretation

Functional iron deficiency shows symptoms (fatigue, restless legs, exertional dyspnea) at ferritin 30–60 even without anemia; many functional clinicians treat to ≥70–100.

Research note

Ferritin glycation, isoform composition (H vs L chains), and intracellular ferritin localization are active research areas; serum ferritin is a rough proxy.

Differential

Causes of abnormal values

Causes of HIGH
  • Inflammation / infection (acute-phase response)
  • Iron overload (hereditary hemochromatosis, transfusional)
  • Liver disease (release from hepatocytes)
  • Malignancy
  • Adult-onset Still's disease (extreme elevations >10 000)
  • Metabolic syndrome / NAFLD
  • Daily alcohol use
Causes of LOW
  • Iron deficiency (always — there is no false low)
  • Hypothyroidism (mild)
Multi-marker patterns

Pattern recognition

Low ferritin alone

Iron deficiency. Investigate source: GI loss in adults of either sex, menstrual loss in premenopausal women.

High ferritin + high TSAT (>45%)

Iron overload — HFE genotype, MRI T2*, consider phlebotomy.

High ferritin + normal TSAT + high CRP

Inflammation, not overload. Treat the cause; do not phlebotomize.

Pitfalls

Pre-analytic & interpretation traps

  • !Ferritin <30 µg/L is essentially diagnostic of iron deficiency; <100 in inflammation/heart failure can still represent deficiency.
  • !BRITISH SOCIETY OF GASTROENTEROLOGY uses <30 µg/L; in chronic inflammation thresholds rise to <100 µg/L.
  • !Heterophile antibodies and rheumatoid factor can falsely elevate immunoassay ferritin.
Follow-up orders

Logical next-step labs

Evidence-graded claims

What the data says

A
Ferritin <30 µg/L is essentially diagnostic of absolute iron deficiency
Multiple guidelines (BSG, AGA, WHO).
B
Iron supplementation improves restless legs syndrome when ferritin <75
RLS guidelines support IV iron when ferritin <75 and TSAT <20%.
C
Targeting ferritin to 'optimal' 70–100 in non-anemic patients improves general fatigue
Plausible, several small RCTs positive in women; not universally replicated.
B
Routine ferritin screening detects pre-clinical hemochromatosis
Combined with TSAT and HFE genotype; cost-effective in high-prevalence populations.
References

Primary literature

Related

Related biomarkers